Complete amino acid sequence of jack bean urease.
نویسندگان
چکیده
منابع مشابه
The amino acid composition of jack-bean urease.
Analyses were carried out on twice-recrystallized freeze-dried urease (Bailey & Boulter, 1969) that had previously been dialysed for 24hr. in the cold against running tap water, and then for 24hr. against three changes of deionized water. Acid hydrolysis of urease (5mg.) was carried out at 1100 in evacuated heavy-walled Pyrex tubes with threetimes-redistilled 5-7M-hydrochloric acid at a concent...
متن کاملKinetics and mechanism of jack bean urease inhibition by Hg2+
UNLABELLED BACKGROUND Jack bean urease (EC 3.5.1.5) is a metalloenzyme, which catalyzes the hydrolysis of urea to produce ammonia and carbon dioxide. The heavy metal ions are common inhibitors to control the rate of the enzymatic urea hydrolysis, which take the Hg2+ as the representative. Hg2+ affects the enzyme activity causing loss of the biological function of the enzyme, which threatens ...
متن کاملIx. the Half-unt and Hempolymers of Jack Bean Urease*
Investigation of their ultracentrifugal and electrophoretic behavior and chemical reactivity has been used to characterize the suprastructure of the nongenetic urease isozymes. As purified from jack bean meal, urease is obtained in the LY form (M, = 480,000, s20,W = 18.3), which is composed of two noncovalently bound, equal and active A1 half-units (M, = 240,000, sZo,W = 11.5) which separate af...
متن کاملThe nickel ion environment in jack bean urease.
Preliminary results of an extended X-ray absorption fine structure (e.x.a.f.s.) and X-ray absorption near edge structure study of jack bean urease have recently been reported [Hasnain & Piggott (1983) Biochem. Biophys. Res. Commun. 112, 279]. These results indicate that the environment of the nickel ion in the enzyme is similar to that in the model compounds Ni(L)2(L')1(ClO4)1 (where L is 1-n-p...
متن کاملJack bean (Canavalia ensiformis) urease. Probing acid-base groups of the active site by pH variation.
A pH-variation study of jack bean (Canavalia ensiformis) urease steady-state kinetic parameters and of the inhibition constant of boric acid, a urease competitive inhibitor, was performed using both noninhibitory organic (MES, HEPES and CHES) and inhibitory inorganic (phosphate) buffers, in an effort to elucidate the functions exercised in the catalysis by the ionizable groups of the enzyme act...
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ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1985
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.61.395